- PII
- 10.31857/S0026365624020268-1
- DOI
- 10.31857/S0026365624020268
- Publication type
- Status
- Published
- Authors
- Volume/ Edition
- Volume 93 / Issue number 2
- Pages
- 239-243
- Abstract
- Nonsense mutations in the essential SUP45 and SUP35 genes, encoding translation termination factors, affect the viability of Saccharomyces cerevisiae cells. Flow cytometry revealed that the viability of mutants was 3.5‒4 times lower compared to the wild-type. Moreover, the mutants were found to have higher sensitivity to ultrasonic treatment.
- Keywords
- SUP45 SUP35 нонсенс-мутации проточная цитофлуориметрия жизнеспособность Saccharomyces cerevisiae
- Date of publication
- 15.03.2024
- Year of publication
- 2024
- Number of purchasers
- 0
- Views
- 48
References
- 1. Журавлева Г.А., Бондарев С.А., Землянко О.М., Москаленко С.Е. Роль белков, взаимодействующих с факторами терминации трансляции eRF1 и eRF3, в регуляции трансляции и прионизации // Мол. биология. 2022. Т. 56. С. 206–226.
- 2. https://doi.org/10.31857/S002689842201013X
- 3. Alexandrov A., Grosfeld E., Mitkevich O., Bidyuk V., Nostaeva A., Kukhtevich I., Schneider R., et al. Systematic identification of yeast mutants with increased rates of cell death reveals rapid stochastic necrosis associated with cell division // bioRxiv. 2021.
- 4. https://doi.org/10.1101/2021.10.20.465133
- 5. Barbitoff Y., Matveenko A., Matiiv A., Maksiutenko E., Moskalenko S., Drozdova P., Polev D., Beliavskaia A., Danilov L., Predeus A., Zhouravleva G. Chromosome-level genome assembly and structural variant analysis of two laboratory yeast strains from the Peterhof Genetic Collection lineage // G3: Genes, Genomes, Genetics (Bethesda). 2021. V. 11.
- 6. https://doi.org/10.1093/g3journal/jkab029
- 7. Chabelskaya S., Kiktev D., Inge-Vechtomov S., Philippe M., Zhouravleva G. Nonsense mutations in the essential gene SUP35 of Saccharomyces cerevisiae are non-lethal // Mol. Genet. Genoms. 2004. V. 272. P. 297–307. https://doi.org/10.1007/s00438-004-1053-1
- 8. Davey H., Guyot S. Estimation of microbial viability using flow cytometry // Curr. Protoc. Cytom. 2020. V. 93. Art. e72. https://doi.org/10.1002/cpcy.72
- 9. Gietz R., Schiestl R., Willems A., Woods R. Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure // Yeast. 1995. V. 11. P. 355‒360. https://doi.org/10.1002/yea.320110408
- 10. Inge-Vechtomov S., Zhouravleva G., Philippe M. Eukaryotic release factors (eRFs) history // Biol. Cell. 2003. V. 95. P. 195–209.
- 11. https://doi.org/10.1016/s0248-4900 (03)00035-2
- 12. Kaiser C., Michaelis S., Mitchell A. Spring Harbor laboratory course manual. NY: Cold Spring Harbor Laboratory Press, 1994. 234 p.
- 13. Kwolek-Mirek M., Zadrag-Tecza R. Comparison of methods used for assessing the viability and vitality of yeast cells // FEMS Yeast Res. 2014. V. 14. P. 1068–1079.
- 14. https://doi.org/10.1111/1567-1364.12202
- 15. Maksiutenko E., Barbitoff Y., Matveenko A., Moskalenko S., Zhouravleva G. Gene amplification as a mechanism of yeast adaptation to nonsense mutations in release factor genes // Genes (Basel). 2021. V. 12. Art. 2019. https://doi.org/10.3390/genes12122019
- 16. Merritt G., Naemi W., Mugnier P., Webb H., Tuite M., von der Haar T. Decoding accuracy in eRF1 mutants and its correlation with pleiotropic quantitative traits in yeast // Nucl. Acids Res. 2010. V. 38. P. 5479–5492.
- 17. https://doi.org/10.1093/nar/gkq338
- 18. Moskalenko S., Chabelskaya S., Philippe M., Inge-Vechtomov S., Zhouravleva G. Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae // BMC Mol. Biol. 2003. V. 4.
- 19. https://doi.org/10.1186/1471-2199-4-2
- 20. RStudio Team (2020). RStudio: Integrated Development for R. RStudio, PBC, Boston, MA.
- 21. URL: http://www.rstudio.com/
- 22. Sambrook J., Fritsch E., Maniatis T. Molecular cloning: a laboratory manual // 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989. 1659 p.
- 23. Valouev I., Kushnirov V., Ter-Avanesyan M. Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulation // Cell Motil. Cytoskeleton. 2002. V. 52. P. 161–173. https://doi.org/10.1002/cm.10040
- 24. Volkov K., Aksenova A., Soom M., Osipov K., Svitin A., Kurischko C., Shkundina I., Ter-Avanesyan M., Inge-Vechtomov S., Mironova L. Novel non-mendelian determinant involved in the control of translation accuracy in Saccharomyces cerevisiae // Genetics. 2002. V. 160. Р. 25‒36.
- 25. https://doi.org/10.1093/genetics/160.1.25
- 26. Zhouravleva G., Frolova L., Le Goff X., Le Guellec R., Inge-Vechtomov S., Kisselev L., Philippe M. Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3 // EMBO J. 1995. V. 14. P. 4065–4072.
- 27. https://doi.org/.1002/j.1460-2075.1995.tb00078.x
