- PII
- 10.31857/S0026365624050168-1
- DOI
- 10.31857/S0026365624050168
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 93 / Issue number 5
- Pages
- 666-670
- Abstract
- A technology has been developed for producing a probiotic preparation by solid-state cultivation of a mixed culture of Bacillus subtilis B-8130, B. subtilis B-2984, B. subtilis B-4099 on beet pulp, which is shown in two preparations (ProStor and GerbaStor). Solid-state fermentation was carried out under conditions of limited access to oxygen at a temperature of 40 ± 5°C, pH 7.5‒8.0 and mixture humidity 45 ± 3% for 48‒50 hours. During the process, acidification of the reaction mass was observed, a significant increase in cell titer and the formation of a biofilm on phytosorbent. The cell titer depended on the factor “stage of fermentation”, but did not depend on the factor type of preparation ‒ ProStor or GerbaStor. Among the identified volatile products, by 48 hours of fermentation the content of acetic acid, 3-methylbutanoic acid, p-guaiacol and 2-n-pentylfuran increased, and aromatic substances of the phenolic series decreased. Additional metabolites were obtained that enhance the antibacterial properties and taste of the probiotic preparation. The formation of a biofilm made it possible to preserve viable cells during drying (45°C): in ProStor 1.2 × 108 CFU/g, and in GerbaStor 0.58 × 108 CFU/g. In HerbaStore with an expanded range of medicinal plants, a decrease in the titer of bacilli is associated with dehydration during drying and with the antibacterial properties of phytocomponents.
- Keywords
- пробиотик Bacillus subtilis твердофазная ферментация биопленка метаболиты твердофазной ферментации
- Date of publication
- 15.09.2024
- Year of publication
- 2024
- Number of purchasers
- 0
- Views
- 33
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