- PII
- S3034546425020062-1
- DOI
- 10.7868/S3034546425020062
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 94 / Issue number 2
- Pages
- 179-184
- Abstract
- Abstract. Minor zinc-dependent metalloendopeptidase secreted by the soil strain Bacillus pumilus 3-19 occupies a unique intermediate classification position between two families of the metzincin clan: adamalizins and astacins. To be able to study the functional role of metalloendopeptidase in more detail, it is necessary to obtain a pure protein preparation in sufficient quantity. Since the enzyme secretion by the B. pumilus 3-19 strain is extremely low, recombinant strains producing metalloendopeptidase MprBp were constructed based on protease-free strains of Bacillus subtilis, as well as methylotrophic yeast Pichia pastoris. The obtained strains were evaluated for the efficiency of metalloendopeptidase production. The B. subtilis strain BG2036+mprBp had the highest production of the target protein.
- Keywords
- металлоэндопептидаза продукция белка рекомбинантный штамм Bacillus pumilus Bacillus subtilis Pichia pastoris
- Date of publication
- 01.04.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 57
References
- 1. Altenbuchner J. Editing of the Bacillus subtilis genome by the CRISPR-Cas9 system // Appl. Environ. Microbiol. 2016. V. 82. P. 5421–5427.
- 2. Bond J. S. Proteases: history, discovery, and roles in health and disease // J. Biol. Chem. 2019. V. 294. Р. 1643–1651.
- 3. Danilova Y. V., Rudakova N. L., Vasilyeva Y. A., Gilmutdinova A. I., Diadkina I. V., Khasanov D. I., Sharipova M. R. Optimization of electroporation conditions for Bacullus pumilus 3-19 strain // BioNanoSci. 2022. V. 12. P. 752–756.
- 4. Demidyuk I. V., Romanova D. V., Nosovskaya E. A., Chestukhina G. G., Kuranova I. P., Kostrov S. V. Modification of substrate-binding site of glutamyl endopeptidase from Bacillus intermedius // Prot. Eng. Des. Sel. 2004. V. 17. P. 411–416.
- 5. Huang J., Pan Y., Hu G., Sun W., Jiang L., Wang P., Ding X. SRC fine-tunes ADAM10 shedding activity to promote pituitary adenoma cell progression // FEBS J. 2020. V. 287. P. 190–204.
- 6. Khasanov D. I., Rudakova N. L., Koryagina A. O., Sharipova M. R. CRISPR/Cas9-redacted Bacillus subtilis strain as the producer of extracellular metalloproteinase of B. pumilus // Opera Medica et Physiologica. 2022. V. 9. № 3. P. 121–127.
- 7. Madden K., Tolstorukov I., Cregg J. Electroporation of Pichia pastoris // Genetic transformation systems in fungi. V. 1. Fungal Biology / Eds. van den Berg M., Maruthachalam K. Cham: Springer, 2015. P. 87–91. https://doi.org/10.1007/978-3-319-10142-2_8
- 8. MEROPS the Peptidase Database. Release 12.5. https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=M12. Проверено 15.10.2024.
- 9. PichiaPinkTM Expression System (“Invitrogen”) manual. http://tools.thermofsher.com/content/sfs/manuals/pichiapink_expression_system_man.pdf. Проверено 13.10.2024.
- 10. Pudova D. S., Toymentseva A. A., Gogoleva N. E., Shagimardanova E. I., Mardanova A. M., Sharipova M. R. Comparative genome analysis of two Bacillus pumilus strains producing high level of extracellular hydrolases // Genes. 2022. V. 13. Art. 409.
- 11. Rudakova N. L., Sabirova A. R., Khasanov D. I., Danilova I. V., Sharipova M. R. Regulating pathways of Bacillus pumilus adamalysin-like metalloendopeptidase expression // Int. J. Mol. Sci. 2024. V. 25. P. 1–9.
- 12. Sabirova A. R., Rudakova N. L., Balaban N. P., Ilyinskaya O. N., Demidyuk I. V., Kostrov S. V., Rudenskaya G. N., Sharipova M. R. A novel secreted metzincin metalloproteinase from Bacillus intermedius // FEBS Letters. 2010. V. 584. P. 4419–4425.
- 13. Serrano-Garrido O., Peris-Torres C., Redondo-García S., Asenjo H. G., Plaza-Calonge M.D.C., Fernandez-Luna J.L., Rodriguez-Manzaneque J.C. ADAMTS1 supports endothelial plasticity of glioblastoma cells with relevance for glioma progression // Biomolecules. 2020. V. 11. Art. 44.
- 14. Shen X., Chen Y., Liu T., Hu X., Gu Z. Development of a high-efficient transformation system of Bacillus pumilus strain DX01 to facilitate gene isolation via gfp-tagged insertional mutagenesis and visualize bacterial colonization of rice roots // Folia Microbiol. (Praha). 2013. V. 58. P. 409–417.
- 15. Steel R. G.D., Torrie J. H., Dicky D. A. Principles and procedures of statistics: a biometrical approach. New York: McGraw Hill, Inc. Book Co., 1997. 666 p. 3rd ed.
- 16. Toymentseva A. A., Danilova I. V., Tihonova A. O., Sharipova M. R., Balaban N. P. Purification of recombinant extracellular proteases from Bacillus pumilus for β-amyloid peptide cleavage // Russ. J. Bioorg. Chem. 2016. V. 42. P. 62–68.
- 17. Zhong S., Khalil R. A. A Disintegrin and Metalloproteinase (ADAM) and ADAM with thrombospondin motifs (ADAMTS) family in vascular biology and disease // Biochem. Pharmacol. 2019. V. 164. P. 188–204.
