- PII
- 10.31857/S0026365624050156-1
- DOI
- 10.31857/S0026365624050156
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 93 / Issue number 5
- Pages
- 662-665
- Abstract
- The search for new amyloid proteins, as well as the study of their properties, is an actual task, which can be solved by a number of different model systems. One of the most popular is the C-DAG approach. It is based on the analysis of aggregation of the investigated proteins on the surface of Escherichia coli cells. According to the original protocol, it can be used to demonstrate one of the characteristic properties of amyloids: the ability to bind the amyloid-specific dye Congo red and demonstrate apple-green birefringence. In addition, the C-DAG technique allows one to analyze the morphology of aggregates and their resistance to detergents. In this work, we tested using Sup35NM as an example whether aggregates on the surface of bacterial cells can act as inducers of aggregation of the corresponding protein.
- Keywords
- амилоиды C-DAG E. coli кинетика агрегации Sup35
- Date of publication
- 15.09.2024
- Year of publication
- 2024
- Number of purchasers
- 0
- Views
- 34
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