RAS BiologyМикробиология Microbiology

  • ISSN (Print) 0026-3656
  • ISSN (Online) 3034-5464

Inactivation of the gene responsible for the synthesis of gluconic acid in the genome of Pantoea brenneri

PII
S3034546425020095-1
DOI
10.7868/S3034546425020095
Publication type
Status
Published
Authors
Volume/ Edition
Volume 94 / Issue number 2
Pages
199-202
Abstract
Abstract. To study the contribution of the glucose dehydrogenase (gcd) gene product to the development of ISR priming in plants, a strain of Pantoea brenneri 3.2 with a deletion of the gcd gene was obtained. Using the Lambda Red (λ Red) phage recombination system, we obtained a marker-free mutant strain of P. brenneri 3.2 Δgcd. Inactivation of the gcd glucose dehydrogenase gene resulted in a 2.5-fold decrease in the strain’s ability to solubilize tricalcium phosphate on the solid nutrient medium NBRIP.
Keywords
ISR-прайминг солюбилизация фосфатов Pantoea brenneri
Date of publication
01.04.2025
Year of publication
2025
Number of purchasers
0
Views
62

References

  1. 1. Бульмакова Д. С., Шагиева Г. И., Иткина Д. Л., Ленина О. А., Шарипова М. Р., Сулейманова А. Д. Антагонистические штаммы Pantoea brenneri как средства защиты растений // Микология и фитопатология. 2023. Т. 57. C. 352–361.
  2. 2. Иткина Д. Л., Сулейманова А. Д., Шарипова М. Р. Pantoea brenneri AS3 и Bacillus ginsengihumi M2.11 как потенциальные агенты биоконтроля и стимуляторы роста растений // Микробиология. 2021. Т. 90. С. 204–214.
  3. 3. Itkina D. L., Suleimanova A. D., Sharipova M. R. Pantoea brenneri AS3 and Bacillus ginsengihumi M2.11 as potential biocontrol and plant growth-promoting agents // Microbiology (Moscow). 2021. V. 90. P. 210–218.
  4. 4. Datsenko K. A., Wanner K. A. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products // Proc. Natl. Acad. Sci. USA. 2000. V. 97. P. 6640–6645.
  5. 5. Hanahan D. Studies on transformation of Escherichia coli with plasmids // J. Mol. Biol. 1983. V. 166. P. 557–580.
  6. 6. Kumar A., Verma J. Does plant-microbe interaction confer stress tolerance in plants: a review // Microbiol. Res. 2018. V. 207. P. 41–52.
  7. 7. Park J. H., Bolana N., Megharaj M., Naidua R. Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil // J. Hazard. Mater. 2011. V. 185. P. 829–836.
  8. 8. Paul D., Sinha S. N. Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from river Ganga, India // Ann. Agrar. Sci. 2017. V. 15. P. 130–136.
  9. 9. Rawat P., Das S., Shankhdhar D., Shankhdhar S. C. Phosphate-solubilizing microorganisms: mechanism and their role in phosphate solubilization and uptake // J. Soil Sci. Plant Nutr. 2020. V. 21. P. 49–68.
  10. 10. Sambrook J., Fritsch E. F., Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. NY: Cold Spring Harbor Laboratory Press, 1989.
  11. 11. Suleimanova A. D., Beinhauer A., Valeeva L. R., Chastukhina I. B., Balaban N. P., Shakirov E. V., Greiner R., Sharipova M. R. Novel glucose-1-phosphatase with high phytase activity and unusual metal ion activation from soil bacterium Pantoea sp. strain 3.5.1 // Appl. Environ. Microbiol. 2015. V. 81. P. 6790–6799.
  12. 12. Suleimanova A., Bulmakova D., Sokolnikova L., Egorova E., Itkina D., Kuzminova O., Gizatullina A., Sharipova M. Phosphate solubilization and plant growth promotion by Pantoea brenneri soil isolates // Microorganisms. 2023. V. 11. Art. 1136. https://doi.org/10.3390/microorganisms11051136
QR
Translate

Индексирование

Scopus

Scopus

Scopus

Crossref

Scopus

Higher Attestation Commission

At the Ministry of Education and Science of the Russian Federation

Scopus

Scientific Electronic Library