- PII
- S3034546425040045-1
- DOI
- 10.7868/S3034546425040045
- Publication type
- Article
- Status
- Published
- Authors
- Volume/ Edition
- Volume 94 / Issue number 4
- Pages
- 351-355
- Abstract
- We have mapped RNA-DNA hybrids in the prokaryotic genome for the first time. Using the S9.6 antibody immunoprecipitation method (S9.6-DRIP) followed by whole-genome sequencing, we identified 219 unique peaks of RNA-DNA hybrids in the genome of Escherichia coli TOP10. These peaks corresponded to 219 different genes and were predominantly distributed in the coding regions of the genome (88.12%). Analysis of individual genes containing RNA-DNA hybrids revealed that they encode enzymes involved in important energy and metabolic processes in prokaryotes, such as lipoic acid synthesis.
- Keywords
- Escherichia coli ТОР10 РНК-ДНК гибриды (R-петли) антитела S9.6 метод S9.6-DRIP
- Date of publication
- 01.08.2025
- Year of publication
- 2025
- Number of purchasers
- 0
- Views
- 64
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